For the primary colorectal cancer, the low expression of ADCY5 was significantly associated with longer survival (Fig 6B), whereas no statistically significant differences were found for the corresponding ADRA2A and EEF2 cohorts (Fig 6C and 6D), which may be due to the limited numbers of patients recorded in TCGA (n = 264) that were not sufficient to distinguish the two cohorts of the complex CRC disease. This evidence concerns the gene ADCY5 and colorectal cancer.