Only in the infected mice, we detected neutrophils included within cluster 30 (Siglec-Hlow PD-L1- CD86-) (Fig 1E), this subpopulation was reduced at 48 and 72 h post infection (p<0.05, between the numbers at 48 and 72 h post infection versus the 24 h post infection; one-way ANOVA). Here, CD86 is linked to infection.