In summary, in this study, it was tentatively hypothesized that LTBP2 might activate the unconventional NF-κB pathway by regulating NF-κB2 gene expression, thereby inducing cisplatin resistance in GC cells, and further experiments were conducted to demonstrate this in combination with the HGC-27 cisplatin-resistant cell line. This evidence concerns the gene LTBP2 and gastric cancer.