To obtain a more comprehensive and complete information, we analyzed 25 ferroptosis related genes (including MT1G, GLS2, NFE2L2, CDKN1A, SAT1, GPX4, HSPA5, ACSL4, SLC7A11, TFRC, EMC2, RPL8, HSPB1, FANCD2, CS, SLC1A5, CARS, ALOX15, ATL1, FDFT1, LPCAT3, CISD1, ATP5MC3, NCOA4, and DPP4) and the differential analysis showed that except for NCOA4, all of these ferroptosis associated genes were significantly differentially expressed in HCC and normal tissues (Figure 5A). This evidence concerns the gene ACSL4 and hepatocellular carcinoma.