To further validate the effects of inhibiting PHGDH in AML, AML cell lines (HL60 and THP1 for FLT3-ITDwt; MV4-11 and MOLM13 for FLT3-ITD+) were treated with different concentrations of the PHGDH inhibitor NCT503. This evidence concerns the gene PHGDH and acute myeloid leukemia.