In the present study, both circ-231 and eIF4A3 were overexpressed in ESCC and participated in TPI1 and PRDX6 mRNA initial translation, suggesting that both circ-231 and eIF4A3 were involved in tumor progression via post-transcriptionally regulating the expression of TPI1 and PRDX6. This evidence concerns the gene PRDX6 and esophageal squamous cell carcinoma.