Subsequently, we evaluated the functional status of CD8+ T cells by analyzing intracellular IFN-γ and granzyme B (GZMB) production and evaluated CD8+ T cell exhaustion using the expression of PD-1 and Tim-3 on the cell surface in tumor-infiltrating lymphocytes (TILs) using flow cytometry (Figure 4). This evidence concerns the gene PDCD1 and neoplasm.