Univariate and multivariate COX regression analyses showed that these five hub genes acted as risk factors in colorectal cancer, with ASB6 demonstrating the most significant association with prognosis (Univariate: HR=2.9, 95% CI: 1.79-4.69; Multivariate: HR=2.35, 95% CI: 1.32-4.19) (Figure 1C). This evidence concerns the gene ASB6 and colorectal cancer.