Additionally, an analysis of cell distribution in glioma using single-cell RNA-seq datasets from UCSC Cell Browser (https://gbm.cells.ucsc.edu) [30, 31] and Single Cell Portal (https://singlecell.broadinstitute.org, GSE131928) [11], revealed that only GPR65 was predominantly expressed on macrophages, while the others showed low expression level or ambiguous cell localizations (Fig. 4D-E & S4E-N). This evidence concerns the gene GPR65 and glioma.