Our data indicate that the TWIST1/Vimentin positivity in urothelial carcinoma cases exceeds that in other urological malignancies (prostate cancer, kidney cancer, urachal carcinoma), with the difference being Interesting, no significant methylation differences were found between urothelial carcinoma cases and upper tract urothelial carcinoma (UTUC) cases, despite their distinct etiologies and pathogenesis, as both are derived from urothelial tumors20. This evidence concerns the gene TWIST1 and renal pelvis/ureter urothelial carcinoma.