Our data indicate that the TWIST1/Vimentin positivity in urothelial carcinoma cases exceeds that in other urological malignancies (prostate cancer, kidney cancer, urachal carcinoma), with the difference being Interesting, no significant methylation differences were found between urothelial carcinoma cases and upper tract urothelial carcinoma (UTUC) cases, despite their distinct etiologies and pathogenesis, as both are derived from urothelial tumors20. The gene discussed is TWIST1; the disease is bladder urachal carcinoma.