Monitoring the BALB/c recipients revealed that the co-transfer of allogeneic B6 CD8+ TN subsets induced GVHD; however, the disease severity was distinct among each subset, with CD5lo cells being worse than Ly6C− and Ly6C+ cells, as evidenced by the maximum loss in body weight and the least survival during the onset of GVHD (Fig. 4b, c). This evidence concerns the gene CD8A and graft versus host disease.