At 72 hpi, LS parasite maturation and hepatic merozoite formation were assessed by quantifying the percentage of monolayer EEFs expressing merozoite surface protein 1 (MSP1) and apical membrane antigen 1 (AMA1) in immunolabeled monolayers; we collected the culture medium from the same wells to quantify merozoite-filled, detached HepG2 cells, the presentation of merosome formation in the P. berghei–HepG2 infection model (30). The gene discussed is ATAD1; the disease is infection.