A T2D case–control analysis, between 113 individuals with T2D and 135 individuals without T2D, identified 3 previously unreported genome-wide significant sites of differential DNA methylation annotated to the body of RGL3 (chr19:11529587), the body of NGB (chr14:77736811) and downstream from OTX2 (chr14:57265055) at FDR 5%. Here, RGL3 is linked to type 2 diabetes mellitus.