In this study, we evaluatedthe binding and uptake of biodegradableFSNs conjugated with CEA antibodies, compared to untargeted PEGylatedFSNs in colon cancer cell lines and tumor tissues of CRC animal models.CEA was used as a target-specific biomarker for directed binding anduptake in CRC. This evidence concerns the gene CEACAM5 and malignant colon neoplasm.