To this end, Acta2-CreERT2, ROSA26R(mTmG/+) mice were induced with 4-OH-TM on P6.5, P8.5 and P10.5, and then rested until P60.5, after which they were subjected to 21 days of normoxia or hypoxia to induce pulmonary hypertension and right ventricle hypertrophy (Figures 4A–C). Here, ACTA2 is linked to pulmonary hypertension.