TGFB1 and neoplasm: Furthermore, since the activation of TGF-β signaling might affect the sorting of molecular cargo from tumor-derived EVs (33), CRC cells (SW480) were kept in the presence of TGF-β for 72 h, and upon EV isolation followed by RNA sequencing, high enrichment of SNHG10 was found relative to EVs derived from non-stimulated cells (80).