As shown in Fig. 8A, the genes with the top 20 mutation frequencies are listed, all of which represent higher mutation frequencies in high-risk group than in low risk group, such as TTN and TP53. Moreover, the TMB of all tumor samples was calculated, and the TMB in the high-risk samples was obviously higher than that in the low-risk specimens (p < 0.05, Fig. 8B). This evidence concerns the gene TP53 and neoplasm.