In our experiments, knockdown of MSLN significantly reduced the migratory and invasive capacity of brain metastatic NSCLC cells (PC9-BrM and H2030-BrM), whereas overexpression of MSLN increased the migratory and invasive capacity of the parental cells (PC9 and H2030, respectively), as evidenced by the wound healing and Transwell assays (Fig. 2A-C, Fig. S3). Here, MSLN is linked to non-small cell lung carcinoma.