EGFR and neoplasm: While ROI-associated transcriptomes of EGFR-positive-, EGFR-negative tumour bearing and control brains revealed several differentially expressed genes (DEGs) between large and small blood vessels, their expression was only partially restricted to endothelial cells and may reveal more global changes in the perivascular microenvironment in EGFR-driven tumours (Supplementary Fig. S9d–f).