Notably, the MMTV-neu model was constructed by ErbB2 oncogene overexpression in mice and was driven by the MMTV promoter (Fig. 5b).238 Studies have validated that the model can generate pre-invasive disease stage that exhibit histological similarities to human DCIS.228,236 Based on this characteristic, Lezzi et al. developed a dynamic mouse model (Balb-NeuT mice) for studying breast cancer progression in vivo. This evidence concerns the gene ERBB2 and ductal breast carcinoma in situ.