In recent years, the incidence of LC has continued to raise, with a 5-year overall survival rate of only 50%, and many patients are already in the advanced stage when discovered.4,5 HPV infection performs a vital function in the progression of LC ,9–11 Our research displayed that HPV transfection augmented the autophagy and growth of LC cells by regulating the NRSN2-mediated activation of the AMPK/ULK1 pathway. The gene discussed is ULK1; the disease is laryngotracheoesophageal cleft.