Traditional biomarker studies largely focus on whole‐exome sequencing (WES) or RNA sequencing (RNA‐seq) of tumor tissues, and these approaches only reflect the average genetic profile of tumors,[4] such as expression of programmed cell death‐ligand 1 (PD‐L1),[5] microsatellite instability (MSI)[6] and the tumor mutation burden (TMB).[5] With the advent of single‐cell RNA sequencing (scRNA‐seq), there are a variety of techniques for analyzing gene expression at the cellular level, facilitating the identification of more sensitive markers. Here, CD274 is linked to neoplasm.