We have further identified that PKA specifically phosphorylates the cytoplasmic TAF15 variant in standard cell lines (e.g. HEK293T cells) and an ALS disease-relevant cell line (e.g. glioblastoma brain cell line) (Supplementary Fig. S5), indicating that this additional regulation layer may be present in ALS pathological conditions. Here, TAF15 is linked to amyotrophic lateral sclerosis.