The results indicated that as the culture time increased, both HepG2 (Figure 6C) and SNU‐387 (Figure 6D) cell lines exhibited significantly lower cell survival in the Si‐SPP1 group than in the control and Si‐Control groups, suggesting that SPP1 might promote the growth of hepatocellular carcinoma cells in some way, consistent with the bioinformatic analysis in our previous study. This evidence concerns the gene SPP1 and hepatocellular carcinoma.