One homozygous, 1 compound heterozygous, and 15 heterozygous MC4R variants were identified upon screening using an obesity gene panel in unrelated adult and pediatric patients of the outpatient clinic Obesity Center CGG, Erasmus MC: D37V, K71N, V95I, I102N, I102T, F149YfsX9, T150I, W174X, I195T, M215R, V253I, V255A, F262L, N274S, C293Y, P299H, and C318Y (Fig. 1 and Table 1). Here, MC4R is linked to obesity due to melanocortin 4 receptor deficiency.