First, we assessed macrophage phagocytosis.Ang-knockout (KO) or wild-type (WT) BMDMs were exposed to FITC-labelled E. coli at a multiplicity of infection (MOI) of 5 or 20 for 1 h, and cellular uptake was assayed by flow cytometry, while bacterial killing was assessed via plate counting. The gene discussed is ANG; the disease is infection.