DLD-1 cells simulated with IL-27 activated the ERK, p38, Akt, STAT1, STAT3, and STAT6 signaling, and increased the expression of alpha-2-macroglobulin (A2M), basic leucine zipper transcriptional factor ATF-like 3 (BATF3), deleted in malignant brain tumor (DMBT1), IDO1, and SOCS3 (140). Here, A2M is linked to brain cancer.