CD276 and metastatic malignant neoplasm in the lymph nodes: Table 1 provides an overview of patients’ clinical features and their CD276 expression. Cox regression analysis was conducted on various potential prognostic factors (age, gender, depth of primary cancer invasion, lymph node metastasis, TNM stages, CD276 expression, tumor site, and differentiation) for overall survival. The results showed that, in ESCC patients following surgery, lymph node metastasis (P=0.018) rather than CD276 expression (P=0.128) was an independent prognostic factor, as demonstrated in Supplementary Table 1.