Then, Deng et al. [8] reported in 2002 that MLH1 methylation correlates with MLH1 expression in a region-specific manner in CRC when using the NaHSO3-digestion method (or the combined bisulfite restriction analysis, COBRA) by using restriction enzyme BstUI that recognizes two consecutive CpG sites (CGCG) from −252 to −249 within this proximal region. The gene discussed is MLH1; the disease is colorectal carcinoma.