MLH1 and colorectal carcinoma: Deng et al. [6] reported in 1999 that methylation of CpG sites in a small region from −269 to −199 before the start codon of the MLH1 gene (Deng Region C, from −248 to −178 relative to the transcription start site in their study) was most associated with the loss of MLH1 expression by using NaHSO3 treatment-sequencing, but it was investigated in CRC cell lines.