To understand the functional involvement of IL3RA in EP300-ZNF384-positive B-ALL cells, we used a doxycycline-inducible shRNA expression system to knock down IL3RA (shIL3RA-1, shIL3RA-2, shIL3RA-3, and shIL3RA-4) in NALM-6 cells. The gene discussed is IL3RA; the disease is acute lymphoblastic leukemia.