These results support that SENP1 acts as deSUMOylation enzyme of AnxA6 to negatively link with AnxA6 expression, and the combination of ANXA6 and SENP1 (ratio of ANXA6/SENP1) would be a potential prognosis biomarker for HCC overall survival. Here, SENP1 is linked to hepatocellular carcinoma.