Based on a relatively low endogenous protein level of AnxA6 in HepG2 and SK-Hep-1 cells, we transiently transfected plasmids pTango-4Flag-AnxA6 (pFlag-AnxA6, ∼ 80 KDa) into these cells to study influence of AnxA6 overexpression on HCC cell migration ability. Here, ANXA6 is linked to hepatocellular carcinoma.