CD4 and neoplasm: Moreover, in another study of single-cell transcriptome data from colorectal cancer in which tumor-infiltrating lymphocytes were profiled along with tumor cells [59], our data showed that the fraction of cancer cells with a high Hypo-MS4-GES was positively correlated with the fraction of regulatory CD4 + T cells (Pearson’s R = 0.46, P = 0.028; Fig. 5C) and negatively correlated with the fraction of tumor-infiltrating CD8+ T cells (Pearson’s R = -0.36, P = 0.087) (Additional file 1: Fig. S11G), both of which promote immune evasion of tumor cells [60, 61].