CL3 Mg-TAMs and Mo co-localized with MES-like states, corresponding to the “Differentiated-like pan-glioma state.” While CL0 Ho-Mg were detected in close proximity to different GBM states, CL1 Ho-Mg were most abundant in close proximity to OPC-like and NPC-like GBM states, equivalent to the “Stem-like pan-glioma states.” This was corroborated by the distribution across spatial GBM niches. Here, ADGRL3 is linked to central nervous system cancer.