The detailed protocol of the FACS isolation protocol is shown in Additional file 2; Fig. 2A. Mean percentage of the CD45+CD11b+ population in the naïve brain was 94.9% microglia/TAMs, 0.6% MonoLy6clow, 0.4% MonoLy6chigh and 3,8% neutrophils, while in the brain with tumor cells we found 75.7% microglia/TAMs, 10.5% MonoLy6clow, 9% MonoLy6chigh and 3.5% neutrophils. The gene discussed is PTPRC; the disease is neoplasm.