To further assess the impact of GPNMB on immune cell composition in the glioma tissue, we stained tumor slices for CD3 labelling T cells in general and combined with Ki-67 labelling the respective proliferating subpopulation, for CD8 labelling CD8+ T cells, for Granzyme B (Gzmb) labelling Granzyme B+ cytotoxic cells and for Foxp3 labelling regulatory T cells. Here, FOXP3 is linked to central nervous system cancer.