As HCC has high intra-tumoral heterogeneity, it becomes especially crucial to detect some hotspot mutations in CTNNB1 genes in tumor protein 53 (TP53), catenin (cadherin-associated protein) beta-1, and telomerase reverse transcriptase (TERT) in ctDNAs [9, 10]. This evidence concerns the gene TP53 and hepatocellular carcinoma.