In addition, immunohistochemical staining was performed on the transplanted tumor of nude mice, and it was found that the expression of HK2 and PKM2 in the transplanted tumor of nude mice could be significantly reduced by knockdown of Lin28B compared with the control group, while simultaneously overexpression of PCAT5 could save the decrease of HK2 and PKM2 (Fig S4E). The gene discussed is PKM; the disease is neoplasm.