IFNG and neoplasm: In contrast to their subpar activity on panned molecular targets, in cocultures with trastuzumab-resistant HER2+ MDA-HER2 cells at a 1:1 effector to target ratio, biotinylated trastuzumab targeted UniCAR T cells secreted IFNγ at amounts comparable to conventional HER2-CAR T cells (Figure 1E; UniCAR+BT or HER2-CAR vs. all other samples: p < 0.001; UniCAR+BT vs. HER2-CAR: n.s.)and also induced killing of monolayer tumor cell cultures with similar potency (Figure 1F; UniCAR+BT or HER2-CAR vs. all other samples: p < 0.01; UniCAR+BT vs. HER2-CAR: n.s. and Supplementary Figure 3).