Since we found that phosphorylation of P65 is upregulated upon one hour of TNF treatment (Fig. 3A-H), whereas, phosphorylation of STAT3 (Fig. 3I-P), translocation of pSTAT3 to the nucleus (Fig. 4K-T) and secretion of both IL6 and IL8 (Fig. 4U-Z) were increased after two hours of TNF stimulation, we stimulated Ctrl and CSMD1-expressing clones of glioma cells with TNF at three different time points: 75 min, 90 min, and 105 min to determine the translocation kinetics of both pP65-Ser536 and total P65. Here, TNF is linked to glioma.