Here, we found that after inhibiting Galectin-3, the mRNA and protein levels of MMP3 in rat cartilage endplate cells decreased, suggesting that Galectin-3 may play a role in intervertebral disc degeneration by regulating the degradation of extracellular matrix in cartilage endplate cells and the expression levels of Aggrecan. The gene discussed is LGALS3; the disease is Intervertebral disk degeneration.