Here, we found that after inhibiting Galectin-3, the mRNA and protein levels of MMP3 in rat cartilage endplate cells decreased, suggesting that Galectin-3 may play a role in intervertebral disc degeneration by regulating the degradation of extracellular matrix in cartilage endplate cells and the expression levels of Aggrecan. This evidence concerns the gene MMP3 and Intervertebral disk degeneration.