This causes excessive ovarian lipid accumulation that results in ovarian lipotoxicity, which promotes ovarian oxidative stress (MDA) by depletion of antioxidant capacity as expressed with decreased level of antioxidant regulator (NrF2) in animals with PCOS compared with control group (Additional file 1: Figure S3b, c). This evidence concerns the gene NFE2L2 and polycystic ovary syndrome.