Thus, we isolated sEV from bladder cancer cells by differential centrifugation, and they displayed sphere-like morphology (Fig. 1A), diameter ~ 100 nm (Fig. 1B), high expression of CD63, Alix, and TSG101, and low expression of the ER-associated chaperone protein calnexin (Fig. 1C). The gene discussed is CD63; the disease is urinary bladder carcinoma.