We used a normal hepatocyte-derived cell line (LO2) as the control and compared the relative expression levels of ASF1A and HJURP in LO2 and five hepatocellular carcinoma (HCC) cell lines (Hep3B, Huh-7, LM3, SNU-368, SNU-739) using qRT‒PCR) and WB experiments. This evidence concerns the gene ASF1A and hepatocellular carcinoma.