Next,to illustrate the generality of our OGTAC strategy, we assessed theeffects of OGTAC-1/2/3 on HTN-Casein kinase alpha (HTN-CK2α).CK2α is a well-documented substrate of OGT, and O-GlcNAcylationon its Ser347 residue was reported to modulate its kinase substrateselectivity.18,19,40 We first coexpressed HTN-CK2α and fOGT in HEK293T cells atvarious ratios and found that the basal O-GlcNAcylation level of HTN-CK2αwas higher than that of HTN-BRD4. Here, BRD4 is linked to hypertensive disorder.