The RL2 signal of HTN-BRD4 displayed a2.5-fold increase at 2 h and further escalated to a 3-fold increaseat 4 h (Figure 2C).However, the effect of OGTAC-1 gradually diminished over longer treatmentdurations, possibly due to the limitation of the transient transfectionsystem: although media with transfection reagents and plasmids wereremoved before OGTAC treatment, the cells continued to express exogenousHTN-BRD4 and fOGT. Here, BRD4 is linked to hypertensive disorder.