This was confirmed to detect Cy3-labeled miR-30a-5p more intuitively by FISH in PCa cell lines transiently and stably transfected with NCAPD3 (Fig. 3C, D), where the fluorescence intensity of miR-30a-5p exhibited a strong negative correlation with the expression of NCAPD3 in all cells. This evidence concerns the gene NCAPD3 and posterior cortical atrophy.