To further validate the role of IQGAP3 in the tumor immune microenvironment, we employed a multi-color fluorescence immunohistochemistry approach to assess the differential expression of IQGAP3, CCR3 (a surface marker for Th2 cells), and CD39 (a surface marker for MDSCs) in human glioblastoma tissue compared to corresponding adjacent non-tumor tissue. The gene discussed is ENTPD1; the disease is glioblastoma.