In the study, we selected and measured the expression of the genes which were reported to correlate with cancer metastasis according to the information of NSCLC tissue sequencing [8] and relevant literature [13–32], and found that the expression levels of IL-17RA, p300, p-STAT3, Ack-STAT3 and MMP19 were significantly higher than other gene in NSCLC samples, suggesting that these proteins may play some roles in NSCLC metastasis. The gene discussed is EP300; the disease is cancer.