MicroRNA regulation promoted suppression of lung cancer cell metastasis by acting on KLFs [37,48] and transcriptional repressor KLF3 regulated miR-182 expression that was increased after knockout of the KLF3 gene using methylation-specific PCR and pyrosequencing techniques, KLF3-DNA hypermethylation and apparent silencing. The gene discussed is KLF3; the disease is lung cancer.