Consistent with the elevated senescence score in osteocytes from breast cancer bone metastasis, we detected an accumulation of senescent osteocytes using a combination of gold standard methods to detect senescence: SA-β-Galactosidase staining, in situ hybridization (p16Ink4a), immunostaining (SASP), and FISH (SADS/TAF) approaches. Here, CDKN2A is linked to breast carcinoma.