Further, cells isolated from bones with breast cancer metastasis exhibited a higher senescence score (constructed with previously reported transcriptional biomarkers of senescence (25, 26)), than from control bones (Fig. 1i) and upregulation of the SASP-related genes Mmp13, Spp1, Serpine2, Timp2, Igfbp7, Igfbp5, and Vegfa. This evidence concerns the gene SPP1 and breast cancer.