We found that compared with DSS treated WT mice, DSS treated Btbd8 KO mice exhibit milder colitis symptoms, such as less body weight loss, decreased Disease Activity Index (DAI) score, and longer colon (Figures 1D–F), whereas no difference in body weight and colonic length between untreated WT and Btbd8 KO mice was observed (Supplementary Figures S1E, F). This evidence concerns the gene BTBD8 and colitis.