BTBD8 and inflammatory bowel disease: To investigate the function of Btbd8 in development and IBD, 1 homozygous and 6 heterozygous Btbd8 knockout (KO) mice were generated by zygotic microinjection of Cas9 and two sgRNAs targeting two sites flanking Btbd8 exon 14 (Figures 1A–C, Supplementary Figures S1A, B).