Remarkably, our cross-region cluster 3 contains individual region clusters with significant negative correlation with AD pathology (ITG cluster 1, PFC cluster 2), yet showed the strongest DAM1 signature (mainly ribosomal response-associated genes), suggesting that these microglia disappear with increasing pathology in human disease, which contrasts with microglial DAM1 phenotype observations in mouse models. The gene discussed is BCAS2; the disease is Alzheimer disease.